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71.
Liqiang Ren Shujie Yang Peiran Zhang Zhiguo Qu Zhangming Mao Po‐Hsun Huang Yuchao Chen Mengxi Wu Lin Wang Peng Li Tony Jun Huang 《Small (Weinheim an der Bergstrasse, Germany)》2018,14(40)
Microfluidic fluorescence‐activated cell sorters (μFACS) have attracted considerable interest because of their ability to identify and separate cells in inexpensive and biosafe ways. Here a high‐performance μFACS is presented by integrating a standing surface acoustic wave (SSAW)‐based, 3D cell‐focusing unit, an in‐plane fluorescent detection unit, and an SSAW‐based cell‐deflection unit on a single chip. Without using sheath flow or precise flow rate control, the SSAW‐based cell‐focusing technique can focus cells into a single file at a designated position. The tight focusing of cells enables an in‐plane‐integrated optical detection system to accurately distinguish individual cells of interest. In the acoustic‐based cell‐deflection unit, a focused interdigital transducer design is utilized to deflect cells from the focused stream within a minimized area, resulting in a high‐throughput sorting ability. Each unit is experimentally characterized, respectively, and the integrated SSAW‐based FACS is used to sort mammalian cells (HeLa) at different throughputs. A sorting purity of greater than 90% is achieved at a throughput of 2500 events s?1. The SSAW‐based FACS is efficient, fast, biosafe, biocompatible and has a small footprint, making it a competitive alternative to more expensive, bulkier traditional FACS. 相似文献
72.
73.
BEPCⅡ加速器的束流位置测量系统(BPM)模拟电子学经过十余年的运行逐渐老化,故障率上升,亟需进行升级改造。本文根据该需求,自行设计了基于BEPCⅡ系统参数的数字BPM电子学系统,内容包括模拟信号处理电子学、数字信号处理电子学、BPM固件算法逻辑、数据获取软件以及系统测试等多个部分。设计的数字BPM电子学系统经实验板级性能测试、实验室系统测试以及在线束流测试,结果表明该系统能满足BEPCⅡ装置对束流位置测量的需求。 相似文献
74.
75.
Yuanming Wu Xiaohua Chen Li Wang Lunjie Wu Lu Lin Wenwu Ding Zhenming Che Jianfeng Wang Jianzhou Li Yi Liu Weifeng Sun 《International Journal of Food Science & Technology》2020,55(4):1825-1832
Preparation of flavour peptides with microorganisms is an attractive choice for its large-scale production. However, beefy meaty peptide (BMP), as a research hotspot in the field of umami peptide, has been studied in few microorganisms, and furthermore, the safe preparation of BMP has not yet been reported. In this study, multi-copy BMP (8BMP) was successfully expressed and produced in the ‘generally recognized as safe’ Bacillus subtilis (B. subtilis). Firstly, 8BMP with the potentially intense umami was screened out based on molecular docking analysis. Then, it was successfully expressed in the B. subtilis 168 and verified through sensory evaluation. Finally, the scaling capacity of 8BMP was evaluated in a 5-L fermenter, with the highest yield (0.84 g L−1) 6.4 times than that of shake flask, which is conductive for industrial production of BMP and other food peptides. 相似文献
76.
Jianjun Chen Min Jiang Wenxin Lin Lijuan Ding Lipeng Xin 《Journal of Materials Science》2018,53(5):3289-3295
Flexible papers constructed by one-dimensional nanowires have attracted much attention due to their various applications. Herein, a novel nonwoven fabric with paper-like qualities composed of zinc blende SiC (β-SiC) nanowires was fabricated by a scalable rolling process. The SiC nanowires were synthesized by the carbothermal reduction reaction of the carbon fiber and carbonaceous silica xerogel. The crystal phase, morphology and microscopic structure of the as-prepared SiC nanowires were characterized by field emission scanning electron microscope, X-ray diffraction and high-resolution transmission electron microscopy. The nanowire vapor–solid growth mechanism and preparation process for SiC nanowire nonwoven fabric were also discussed. The freestanding SiC nanowire nonwoven fabric exhibited high flexibility, high mechanical strength, excellent refractory performance and thermal stability. With high flexibility, high mechanical strength, superior nonflammability and thermal stability, the flexible paper-like 3C-SiC nanowire nonwoven fabric materials would be expected to have some potential applications, such as ceramic matrix composites, metal matrix composites, energy storage, catalyst supports, radiation-proof fabric, filtration and separation. 相似文献
77.
内蒙古某含银铅锌矿主要含有铅、锌、银、铜、硫等有价元素,对该矿进行系统的工艺矿物学研究,查明矿石的矿物组成、结构与构造,目的矿物的嵌布特征、嵌布粒度、赋存状态等,为制定选矿工艺流程提供矿物学依据。 相似文献
78.
热后硬孔攻丝螺纹变形量小、尺寸稳定、强度高。该工艺方法对刀具强度要求较高,使用普通高速钢丝锥和螺纹铣刀加工时,普通高速钢丝锥无法承受热后硬孔攻丝所产生的大扭矩而频繁折断,报废率较高,同时螺纹铣刀成本高,不适合大批量生产。针对热后硬孔攻丝工艺复杂、成本高的问题,专门设计了硬孔攻丝用高性能先端丝锥,解决硬孔攻丝难题,并与其他种类丝锥进行了切削力对比。 相似文献
79.
Chia‐Che Chang Sin‐Ren Li Hung‐Lung Chou Yi‐Cheng Lee Shivaraj Patil Ying‐Sheng Lin Chun‐Chih Chang Yuan Jay Chang Di‐Yan Wang 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(49)
The generation of ammonia, hydrogen production, and nitrogen purification are considered as energy intensive processes accompanied with large amounts of CO2 emission. An electrochemical method assisted by photoenergy is widely utilized for the chemical energy conversion. In this work, earth‐abundant iron pyrite (FeS2) nanocrystals grown on carbon fiber paper (FeS2/CFP) are found to be an electrochemical and photoactive catalyst for nitrogen reduction reaction under ambient temperature and pressure. The electrochemical results reveal that FeS2/CFP achieves a high Faradaic efficiency (FE) of ≈14.14% and NH3 yield rate of ≈0.096 µg min?1 at ?0.6 V versus RHE electrode in 0.25 m LiClO4. During the electrochemical catalytic reaction, the crystal structure of FeS2/CFP remains in the cubic pyrite phase, as analyzed by in situ X‐ray diffraction measurements. With near‐infrared laser irradiation (808 nm), the NH3 yield rate of the FeS2/CFP catalyst can be slightly improved to 0.1 µg min?1 with high FE of 14.57%. Furthermore, density functional theory calculations demonstrate that the N2 molecule has strong chemical adsorption energy on the iron atom of FeS2. Overall, iron pyrite‐based materials have proven to be a potential electrocatalyst with photoactive behavior for ammonia production in practical applications. 相似文献
80.
高光谱激光雷达谱位合一的角度效应分析 总被引:1,自引:0,他引:1
高光谱激光雷达以其谱位合一的技术优势为实现超三维精准遥感观测提供了可行途径,因此成为当前激光雷达与高光谱遥感领域共同大力推进的前沿发展方向。目前已有多型原型系统研发出来并得到了原理性验证,然而针对其数据处理核心环节问题的基础技术仍较为欠缺。典型问题之一是不同波段回波信号受激光入射角度的影响,该角度效应限制了高光谱激光雷达实现高性能遥感。以芬兰空间信息研究所高光谱激光雷达原型系统扫描桦树树干为例探讨了该角度效应,发现了不同激光波段对不同入射角度的回波强度响应模式,推导出了角度效应的基本规律及其精细尺度的统计规律,为后续该方向的系统研发、数据处理及信息提取等提供了可借鉴的底层机理与技术基础。 相似文献